Multiplex PCR Primer DesignBETA

Beta best, typically for SNPs enrichment followed by NGS.
Species.
To avoid primer 3' end sitting on the SNP site.

Primer Design Parameters

MFEprimer Parameters

Min
°C
Minimum Tm value required for the binding stability between primer and its binding sites.
From
To
Product size: 0 (min) - 1000000 (max).