MFEprimer-3.1: PCR Primer Quality Control - Specificity, Hairpins & Dimers

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* Primer sequences (example)

Min loop size

Min

Max loop size

Max

Min double helix size

Min

Allowed max gaps for a hairpin

Max

Score

Min

Score cutoff value to filter hairpins

T_m

Min

°C

T_m cutoff value to filter hairpins

ΔG

Max

kcal/mol

ΔG cutoff value to filter hairpins

Concentration of monovalent cations (usually KCl)

Concentration of divalent cations (usually MgCl₂)

Concentration of dNTPs

Annealing oligo concentration