Dimer

Number of primer sequences: 1 (min) - 50 (max). 2 (max) primers for degenerate bases.
Minimum score value required for a dimer.
How many mismatches allowed in a dimer?
In default, the last 3 bases at the 3'end are treated differently and there should be zero mismatches among these bases.
How many mismatches allowed in 3'end key bases in a dimer?
Maximum continuous mismatches allowed in a dimer.
°C
Temperature for thermodynamics calculation.
mM
mM
mM
nM