MFEprimer-3.1: PCR Primer Quality Control - Specificity, Hairpins & Dimers

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* Primer sequences (example)

Allowed max mismatches for a dimer

Max

Only output dimers with 3'end binded

Score

Min

Score cutoff value to filter dimers

T_m

Min

°C

T_m cutoff value to filter dimers

ΔG

Max

kcal/mol

ΔG cutoff value to filter dimers

Concentration of monovalent cations (usually KCl)

Concentration of divalent cations (usually MgCl₂)

Concentration of dNTPs

Annealing oligo concentration